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Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
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Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Article DOI: 10.3791/56917-v • 10:27 min • February 5th, 2018
February 5th, 2018 •
1,2, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Neurology, Johns Hopkins University, 3Beijing Institute for Brain Disorders, Capital Medical University

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This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

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Zika Virus Infection Human Fetal Brain Neural Stem Cells Immunocytochemical Analysis Viral Infection Innate Immune Responses Survival And Differentiation Translational Application Neurological Deficits Mechanism Of Infection Therapeutics Culture Medium Plating Human Neural Stem Cells Biosafety Cabinet Conditioned Medium Trypsin Inhibitor Solution
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