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Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Article DOI: 10.3791/57134-v • 08:54 min • March 9th, 2018
March 9th, 2018 •
1, 2, 1
1Department of Horticulture, Virginia Tech, 2Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech

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The protocol described herein is a method for measuring endoreduplication within tubers of potato (Solanum tuberosum). It includes plasmolysis and protoplast extraction steps to decrease the noise and debris in downstream flow cytometric analysis.

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Measuring Endoreduplication Flow Cytometry Isolated Tuber Protoplasts Potato Tubers Potato Tuber Morphology Developmental Stage Clearer Results Parker Laimbeer Grad Student Solutions Buffers Surface Sterilize Ethanol Air Dry Conical Tubes Plasmolysis Incubation Solution PIS Sterilized Scalpel Cork Bore Sample Desire To Sterilize Tissue Coarsely Chop Three Cubic Millimeter Pieces Four Degree Celsius Overnight Potato Protoplasts Enzyme Solution Mannitol Calcium Chloride Monopotassium Phosph
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