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Journal / Bioengineering / Optimization of a Multiplex RNA-based Expression…
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Bioengineering

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JoVE Journal Bioengineering
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Article DOI: 10.3791/57148-v • 11:13 min • August 1st, 2018
August 1st, 2018 •
1, 2, 3, 3, 1
1Department of Pathology, Faculty of Medicine & Surgery, University of Malta, 2Centre for Molecular Medicine and Biobanking, University of Malta, 3Department of Physiology & Biochemistry, Faculty of Medicine and Surgery, University of Malta

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Summary
Automatic Translation

Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast cancers.

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Multiplex RNA-based Expression Assay Breast Cancer Archival Material Optimization Therapeutic Groups Sensitivity Laser Microdissection RNA-based Multiplex Assay Reproducibility Hematoxylin And Eosin-stained Formalin-fixed Paraffin-embedded Archival Material High Resolution Outputs Biomarker Validation Tissue Preparation Dissection Slide Limits Microscope Calibration Stained Membrane Slides Target Areas For Microdissection Square Millimeters Downstream RNA Analysis Laser Cutting Cap-lift Mechanism Dissected Section Retrieval Accuracy Of Microdissection
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