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JoVE Journal Chemistry
Essential Metal Uptake in Gram-negative Bacteria
Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
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Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
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Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Article DOI: 10.3791/57169-v • 10:34 min • February 1st, 2018
February 1st, 2018 •
1, 2, 2, 3, 2, 4
1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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Summary
Automatic Translation

A protocol for the extraction of a periplasmic transition metal chaperone in the context of its native binding partners, and biophysical characterization of its substrate contents by X-ray fluorescence and radiometal uptake is presented.

Tags

Metal Uptake Gram-negative Bacteria X-ray Fluorescence Radioisotopes Cell Fractionation Periplasmic Contents Biophysical Characterization X-ray Crystallography Radio Metal Uptake Structure-function Relationships ABC Transport Systems Lipid Bilayer Cross-contamination YfeA Substrate Apo Protein Production Radiotracer Assay Bacterial Subculture Re-suspension
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