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JoVE Journal
Immunology and Infection
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis
 

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Article DOI: 10.3791/57195-v 12:54 min May 19th, 2018
May 19th, 2018

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Summary

This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA analysis.

Tags

Lymph Nodes Large Animals RNA Analysis Transcriptomic Studies Collection Processing Quantitative PCR Reverse Transcription RNA Sequencing Host Immune Responses Pathogen RNA Zoonotic Diseases Livestock Wildlife Species Brucella Abortus Brucellosis E.Coli O157:H7 Intestinal Tracks Tissue Harvesting
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