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Miniaturized Sample Preparation for Transmission Electron Microscopy

Article DOI: 10.3791/57310-v • 09:04 min • July 27th, 2018
July 27th, 2018 •
*1,2, *1, *1,2, 1, 1, 1, 1, 1, 3, 1, 1
1Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, 2Swiss Nanoscience Institute, University of Basel, 3BioEM lab, Biozentrum, University of Basel
* These authors contributed equally

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Summary
Automatic Translation

An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

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Miniaturized Sample Preparation Transmission Electron Microscopy Structure Biology Sensitive Proteins Limited Amount Of Protein Standard Sample Preparation Methods Minute Amounts Of Sample No Harsh Paper Blotting Step Single Cell Analysis Visual Proteomics Protein-related Disease High Resolution Structures Ethane Cup Cryo Box Holder Spider Liquid Nitrogen Ethane Gas Bottle Liquid Ethane Polystyrol Lid Cryogen Container Glow Discharged EM Grid
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