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Journal / Cancer Research / Isolation of Human Endothelial Cells from Normal…
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Cancer Research

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JoVE Journal Cancer Research
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Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Summary
Automatic Translation

Tumor endothelial cells are important determinants of the tumor microenvironment and the course of the disease. Here, a protocol for the isolation of pure and viable endothelial cells from human colorectal carcinoma and normal colon to be used in drug testing and pathogenesis research is described.

Tags

Isolation Human Endothelial Cells Normal Colon Colorectal Carcinoma Protocol Tumor Biology Angiogenesis Blood Vessel System Tumorigenesis Technique Success Rate Tissue Specimen Fresh Scalpel Sterile Forceps Ice-cold HBSS Buffer Antibiotics Centrifuge Tube Wash Fat Removal Necrotic Tissue Parts Cell Culture Petri Dish
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