This Month in JoVE: tuberculosis infection modeling, telemetric temperature pills, bioengineered cartilage, and 3D neuronal networks

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1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production

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    Date Published: 10/01/2015, Issue 104; doi: 10.3791/5750

    Cite this Article

    Chao, W., Kolski-Andreaco, A. This Month in JoVE: tuberculosis infection modeling, telemetric temperature pills, bioengineered cartilage, and 3D neuronal networks. J. Vis. Exp. (104), e5750, doi:10.3791/5750 (2015).


    Here's a look at what's coming up in the October 2015 issue of JoVE: The Journal of Visualized Experiments.

    In JoVE Immunology & Infection, we feature a method for studying tuberculosis (TB), which affects 9 million people and causes 1.5 million deaths every year. The bacteria that cause TB spread through the air and set up infection in the lungs, and may spread to other organs. TB infection is a complex process and difficult to model in vitro. Braian et al. present a novel 3D human lung tissue model that recapitulates the dynamics that occur during TB infection, and provides a useful tool for studying this deadly infectious disease.

    In JoVE Medicine, 80% of the energy used for muscle contraction is released as heat, so physical activity can increase the body's core temperature. If core temperature gets too high, it may decrease physical performance or cause heat-related disorders. So it's important to monitor core temperature during prolonged and strenuous exercise. Bongers et al. describe a telemetric temperature pill that can be swallowed so it noninvasively measures gastrointestinal temperature during exercise. This pill is useful for monitoring core temperatures in laboratory and field-based settings.

    In JoVE Bioengineering, articular cartilage is particularly difficult to repair, and remains an unmet challenge for biomedical engineers. Promising approaches for regenerating cartilage include cell-based therapies. And this month, Smeriglio et al. describe a method for isolating articular chondrocytes and fabricating biomimetic hydrogels. These provide physiologically relevant microenvironments for chondrocyte expansion and maturation. The engineered cartilage tissue can then be assessed for regenerative potential.

    In JoVE Neuroscience, in vitro recordings of electrophysiological activity are mostly done in two-dimensional (2D) neuronal networks. This month, Tedesco et al. present a novel 3D platform that couples neuronal networks to planar micro-electrode arrays (MEAs). Scaffolds made of glass microbeads allow neurons to form complex 3D assemblies. The greater morphological complexity allows a wider range of electrophysiological patterns compared to standard 2D networks, and more closely approximates in vivo neural networks.

    You've just had a sneak peek of the October 2015 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The Journal of Visualized Experiments.


    Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

    Mariateresa Tedesco1, Monica Frega1,2, Sergio Martinoia1, Mattia Pesce3, Paolo Massobrio1

    1Department of Informatics, Bioengineering, Robotics and System Engineering (DIBRIS), University of Genova, 2Donders Institute for Brain, Cognition and Behaviour, Department of Cognitive Neuroscience, Radboud University Medical Center, 3Fondazione Istituto Italiano di Tecnologia (IIT)

    In this work, a novel experimental model in which 3D neuronal cultures are coupled to planar Micro-Electrode Arrays (MEAs) is presented. 3D networks are built by seeding neurons in a scaffold made up of glass microbeads on which neurons grow and form interconnected 3D structures.

    3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

    Piera Smeriglio*1, Janice H. Lai*1,2, Fan Yang1,3, Nidhi Bhutani1

    1Orthopaedic Surgery Department, Stanford University, 2Mechanical Engineering Department, Stanford University, 3Bioengineering Department, Stanford University

    Cartilage repair represents an unmet medical challenge and cell-based approaches to engineer human articular cartilage are a promising solution Here, we describe three-dimensional (3D) biomimetic hydrogels as an ideal tool for the expansion and maturation of human articular chondrocytes.

    A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

    Clara Braian1, Mattias Svensson2, Susanna Brighenti2, Maria Lerm1, Venkata R. Parasa1,2

    1Dept. of Clinical and Experimental Medicine, Linköping University, 2Dept. of Medicine, Karolinska Institute

    Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.

    Using an Ingestible Telemetric Temperature Pill to Assess Gastrointestinal Temperature during Exercise

    Coen C.W.G. Bongers, Maria T.E. Hopman, Thijs M.H. Eijsvogels

    Radboud Institute for Health Sciences, Department of Physiology, Radboud University Medical Center

    This study describes an accurate, reliable and non-invasive technique to continuously measure gastrointestinal temperature during exercise. The ingestible telemetric temperature pill is suitable to measure gastrointestinal temperature in laboratory settings as well as in field based settings.


    No conflicts of interest declared.



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