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Immunology and Infection

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JoVE Journal Immunology and Infection
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
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Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

Article DOI: 10.3791/57574-v • 10:45 min • June 13th, 2018
June 13th, 2018 •
1, 1, 1, *1, *1,2
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, 2Harvard Medical School and Division of Gastroenterology, Boston Children's Hospital
* These authors contributed equally

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Summary
Automatic Translation

Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.

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Deep Dermal Injection Candida Albicans Skin Infection Histological Analyses Immune System Activation Skin Architecture Immune Cells Non-immune Cells Immunological Field Immune Cell Localization Mediators Structural Architecture Of The Skin Fungal Injection Giulia Stamerra PhD Student Professor Marina Vai C.Albicans Strain CAF3-1 Rich Medium Uridine Supplementation Yeast Growth Cellular Concentration Measurement Hyphae Formation
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