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Neuroscience

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JoVE Journal Neuroscience
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Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Article DOI: 10.3791/57648-v • 08:44 min • June 5th, 2018
June 5th, 2018 •
1, 1
1College of Nursing, University of Arizona

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Microglia are brain immune cells that survey and react to altered brain physiology through morphologic changes which may be evaluated quantitatively. This protocol outlines an ImageJ based analysis protocol to represent microglia morphology as continuous data according to metrics such as cell ramification, complexity, and shape.

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Quantifying Microglia Morphology Photomicrographs Immunohistochemistry ImageJ Skeleton Analysis Fractal Analysis High-throughput Sensitive Neuroinflammatory Field Microglia Form And Function Continuous Variables Proprietary Software Screening Cell-by-cell Analysis AnalyzeSkeleton Plugin Fiji 30 Micron Z Stack Iba1 Immunostained Brain Fluorescence Photomicrograph 8-bit Image Gray Scale Conversion DAB Bright Field Photograph FFT Bandpass Filter Plugin
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