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Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector

Article DOI: 10.3791/57652-v • 11:07 min • August 26th, 2018
August 26th, 2018 •
1, 2, 1, 3,4, 5, 5, 6, 7, 2, 1
1Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Austria, 2Institute of Chemical, Environmental and Biological Engineering, TU Vienna, Austria, 3Department of Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Germany, 4Institute for Biochemistry, Biotechnology and Bioinformatics, University of Technology (TU) Braunschweig, Germany, 52nd Zoological Department, Natural History Museum Vienna, Austria, 6Chemical Sciences, Universiti Brunei Darussalam, Brunei, 7Environmental and Life Sciences, Universiti Brunei Darussalam, Brunei

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Summary
Automatic Translation

Minor workers of the ant species Colobopsis explodens are dissected to isolate the wax-like content stored in their hypertrophied mandibular gland reservoirs for subsequent solvent-extraction and analysis by gas chromatography-mass spectrometry. The annotation and identification of volatile constituents using the open-source software MetaboliteDetector is also described.

Tags

Isolation Mandibular Gland Reservoir Bornean Exploding Ants GC-MS MetaboliteDetector Insect Metabolomics Metabolite Profiling Chemical Ecology Volatiles Interaction With Other Organisms Workflow Physical Isolation Un-targeted Profiling Volatile Organic Constituents Biological Sample Dissection Glass Petri Dish Fume Hood Methanol And Water Mixture Storage At Minus 20 Degrees Celsius Short Thread Vials Screw Caps Silicon PTFE Septa Malleable Cold Pack Polystyrene Foam Box Stereo Microscope
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