Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / The Lactate Dehydrogenase Sequestration Assay…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization (Video) | JoVE
Journal (Biology)
Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization (Video) | JoVE
A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
Journal (Biology)
A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
 

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Article DOI: 10.3791/57971-v • 09:34 min • July 27th, 2018
July 27th, 2018 •
*1, *1, 1
1The Autophagy Team, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo
* These authors contributed equally

Chapters

Summary
Automatic Translation

Here a simple and well-validated protocol for measuring bulk autophagic sequestration activity in mammalian cells is described. The method is based on quantifying the proportion of lactate dehydrogenase (LDH) in sedimentable cell fractions compared to total cellular LDH levels.

Tags

Lactate Dehydrogenase Sequestration Assay Autophagic Sequestration Activity Mammalian Cells Bulk Autophagy Cargo Sequestration Endogenous Cargo Overexpression Cargo Probes Paula Szalai Morten Luhr Culture Adherent Cells Tissue Culture Flasks PBS Trypsin EDTA Culture Medium FBS Sterile Tube Trypan Blue Counting Chamber Slide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter