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JoVE Journal
Genetics

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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
 

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Article DOI: 10.3791/58016-v 10:18 min October 16th, 2018
October 16th, 2018

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Summary

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

Tags

Amplification Near Full-length HIV-1 Proviruses Next-generation Sequencing Genetic Composition Cell Types Replication Competent Technique Buffers Lysis Buffer Cell Pellet Thermal Cycler First Round PCR PCR Plate Genomic DNA Tris Hydrochloride Dilution
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