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JoVE Journal Genetics
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Article DOI: 10.3791/58024-v • 10:12 min • June 29th, 2018
June 29th, 2018 •
1,2, 1, 1,2, 1,2, 1,2, 1, 1, 2, 2, 1, 1,2
1Children's Hospital of Eastern Ontario Research Institute, 2Department of Biology, University of Ottawa

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Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.

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High-throughput DNA Extraction Genotyping 3dpf Zebrafish Larvae Fin Clipping Biomedical Field Genetic Models Of Disease Early Identification Of Genotypes High-throughput Method Genetic Epilepsies Genetic Disease Models Mutation Study Transection Of The Larval Fin Dissection Surface Preparation Petri Dish Lid Stereo Microscope P1000 Pipette Tip Three Dpf Larva Anesthetization Process Autoclave Tape
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