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Journal / Genetics / Endogenous Protein Tagging in Human Induced…
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Genetics
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JoVE Journal Genetics
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

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Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

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Endogenous Protein Tagging Human Induced Pluripotent Stem Cells CRISPR/Cas9 Fusion Proteins Fluorescent Tags Knock In Ribonucleoprotein Cas9 Protein CRISPR RNA TracrRNA Donor Plasmid Electroporation FACS Enrichment Clonal Lines Gene Editing Protocol Subcellular Localization Dynamics Regulatory Control Diploid Non-transformed Cells Cardiomyocyte Cells Isogenic Cellular Contexts
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