Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Real Time In Vivo Tracking of Thymocytes in the…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
Journal (Biology)
Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
Constructing Copper Plate and Sapphire Window Assembly (Video) | JoVE
Journal (Biology)
Constructing Copper Plate and Sapphire Window Assembly (Video) | JoVE
 

Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy

Article DOI: 10.3791/58236-v • 08:21 min • October 2nd, 2018
October 2nd, 2018 •
1,2, 3
1School of Medicine and Dentistry, Universidad Católica de Valencia San Vicente Mártir, 2Unidad Mixta CIPF-UCV, Centro de Investigación Príncipe Felipe, 3Department of Medicine, University of Miami

Chapters

Summary
Automatic Translation

The goal of the protocol is to show longitudinal intravital real-time tracking of thymocytes by laser scanning microscopy in thymic implants in the anterior chamber of the mouse eye. The transparency of the cornea and vascularization of the graft allows for continuously recording progenitor cell recruitment and mature T-cell egress.

Tags

Real-time In Vivo Tracking Thymocytes Anterior Chamber Of The Eye Laser Scanning Microscopy Immunology Autoimmunity Immunodeficiency Central Tolerance Thymus Involution Progenitor Recruitment Mature T-cell Egression Vascularized And Grafted Mouse Thymus Segments Development And Function Of The Thymus Pancreatic Islets Kidney Glomeruli Isolate Thymus Sterile Absorbent Paper Towels Dorsal Upright Position Laminar Flow Hood 70% Ethanol Superficial V-shaped Incision Lower Abdomen Thoracic Cavity Dissecting Scissors Chest Incision Ventral Midline Thoracic Cavity Superior Mediastinum
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter