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JoVE Journal
Developmental Biology

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An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
 

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Article DOI: 10.3791/58268-v 10:01 min September 19th, 2018
September 19th, 2018

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Summary

Here, we present a method for generating tissue-specific binary transcription systems in Drosophila by replacing the first coding exon of genes with transcription drivers. The CRISPR/Cas9-based method places a transactivator sequence under the endogenous regulation of a replaced gene, and consequently facilitates transctivator expression exclusively in gene-specific spatiotemporal patterns.

Tags

Tissue-specific Binary Transcription Systems Genome Editing Drosophila Branchless Gene Spatiotemporal Expression Tracheal Airway Epithelium Gene Expression Manipulation Trans Activator GAL4 LexA UAS LexO CRISPR Cas9-based Method
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