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JoVE Journal Neuroscience
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Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Article DOI: 10.3791/58347-v • 08:42 min • October 4th, 2018
October 4th, 2018 •
1, 2, 1
1Department of Physiology and Cell Biology, School of Medicine, University of Nevada, 2Department of Pharmacology, Larner College of Medicine, University of Vermont

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Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.

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Ex Vivo Imaging Cell-specific Calcium Signaling Tripartite Synapse Mouse Diaphragm Neuromuscular Field Muscle Cells Schwann Cells Nerve Stimulation Transgenic Mice Genotype Transversely Section Iridectomy Scissors Rib Cage Vertebral Column Diaphragm Sample Krebs-Ringer Solution Fluorescently Labeled Alpha Bungarotoxin Incubate Minutien Pins Immobilize Diaphragm Silicone Dielectric Gel Oxygenated Krebs-Ringer Solution Microscope Stage
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