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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Article DOI: 10.3791/58612-v • 06:12 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Center for Food Safety

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Summary
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Here, we present a protocol to prepare DNA samples from food and environmental microbiomes for concerted detection and subtyping of Salmonella through quasimetagenomic sequencing. The combined use of culture enrichment, immunomagnetic separation (IMS), and multiple displacement amplification (MDA) allows effective concentration of Salmonella genomic DNA from food and environmental samples. 

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Quasi-metagenomic Analysis Salmonella Detection Genetic Fingerprinting Turnaround Time Food Samples Environmental Samples Laboratory Blender Bag Enrichment Broth Centrifuge MDA Kit Anti-Salmonella
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