Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Robust Comparison of Protein Levels Across…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
Journal (Biochemistry)
DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
Journal (Biochemistry)
Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
 

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Article DOI: 10.3791/59438-v • 08:13 min • April 9th, 2019
April 9th, 2019 •
1,2, *1,2, *1,2, *1,2, 1, 1,2, 1,2
1Centre for Discovery Brain Sciences, University of Edinburgh, 2Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh
* These authors contributed equally

Chapters

Summary
Automatic Translation

This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.

Tags

Quantitative Western Blotting Protein Levels Tissues Development Fluorescent Total Protein Stain Internal Loading Control Snap-frozen Samples Protein Extraction Centrifugation Protein Concentration Bovine Serum Albumin Standards 96-well Optical Plate Absorption Measurement
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter