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Journal / Bioengineering / Optical Imaging of Isolated Murine Ventricular…
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Bioengineering

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JoVE Journal Bioengineering
Optical Imaging of Isolated Murine Ventricular Myocytes
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Optical Imaging of Isolated Murine Ventricular Myocytes

Article DOI: 10.3791/60196-v • 11:32 min • January 17th, 2020
January 17th, 2020 •
1,2, 3, 3, 3, 3, 1, 3, 3
1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center

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Summary
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We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements.

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Optical Imaging Isolated Murine Ventricular Myocytes Heart Failure Cardiac Metabolism Excitation-contraction Coupling Metabolites Cardiac Myocyte Isolation Fluorescent Probe Phenotypic Assessments Cardiac Toxicity Cellular Level Myocyte Function Therapeutics For Heart Failure Transgenic Mice Gene Probing Curing Heart Failure Matthew Klos Shreyas Suresh Laminin Solution Coverslip Attachment
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