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Immunology and Infection

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High Throughput Co-culture Assays for the Investigation of Microbial Interactions
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High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Article DOI: 10.3791/60275-v • 07:00 min • October 15th, 2019
October 15th, 2019 •
1, 1, 2, 1, 1
1Department of Bacteriology, University of Wisconsin-Madison, 2Independent Scholar

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Summary
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The co-culture interaction assays presented in this protocol are inexpensive, high throughput, and simple. These assays can be used to observe microbial interactions in co-culture, identify interaction patterns, and characterize the inhibitory potential of a microbial strain of interest against human and environmental pathogens.

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High Throughput Co-culture Assays Microbial Interactions Microbiome Research Antimicrobial Discovery Laboratory Conditions Technique Bioassay Plates Overgrowth Contamination Overnight Cultures BHI Broth Inoculating Loop Vortexing
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