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Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis
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Preparation of Mitochondria from Ovarian Cancer Tissues and Control Ovarian Tissues for Quantitative Proteomics Analysis

Article DOI: 10.3791/60435-v • 11:51 min • November 18th, 2019
November 18th, 2019 •
1,2,3,4, 5, 1,2,3, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 5Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University

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Summary
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This article presents a protocol of differential-speed centrifugation in combination with density gradient centrifugation to separate mitochondria from human ovarian cancer tissues and control ovarian tissues for quantitative proteomics analysis, resulting in a high-quality mitochondrial sample and high-throughput and high-reproducibility quantitative proteomics analysis of a human ovarian cancer mitochondrial proteome.

Tags

Mitochondria Ovarian Cancer Tissues Control Ovarian Tissues Quantitative Proteomics Analysis Mitochondrial Changes Isolate And Purify Mitochondria Large-scale Proteomics Analysis Energy Metabolism Cell Signaling Oxidative Stress Molecular Mechanism Biomarkers Therapeutic Targets Differential-speed Centrifugation Density Gradient Centrifugation High Quality Mitochondria Samples ITRAQ Quantitative Proteomics Ovarian Cancer Tissue Analysis Mitochondria Proteomics Trypsin Control Tissue Homogenization Density Gradient Medium Mitochondrial Isolation Buffer
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