The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
Authors
The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
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Department of Neurology, University of California, Los Angeles
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Mostany, R., Portera-Cailliau, C. A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window. J. Vis. Exp. (12), e678, doi:10.3791/678 (2008).
फ्लोरोसेंट dextrans रंगों की पूंछ नस इंजेक्शन
रक्त प्रवाह का उपयोग कर दो photon माइक्रोस्कोपी इमेजिंग (कुल अवधि प्रति सत्र 30-60 मिनट, imaged जहाजों की संख्या के आधार पर)
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Cortical रक्त प्रवाह की गतिशीलता इमेजिंग फ्लोरोसेंट dextran रंजक दो photon माइक्रोस्कोपी के साथ कृन्तकों की पूंछ नस में इंजेक्शन द्वारा vivo में अध्ययन किया जा सकता है. यह वीडियो कैसे छवि चूहों के एक गिलास कवर कपाल विंडो तैयारी के माध्यम से neocortex में रक्त प्रवाह की गतिशीलता के लिए एक विधि दिखाया.
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| Name | Type | Company | Catalog Number | Comments |
| Fluorescein isothio-cyanate-dextran | Reagent | Sigma-Aldrich | FD500S | mol wt 500,000 |
| Rhodamine B isothio-cyanate-dextran | Reagent | Sigma-Aldrich | R9379 | mol wt ~70,000 |
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