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Journal / Biology / Single Cell Elektroporatie in vivo binnen de…
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Single Cell Elektroporatie in vivo binnen de Intact ontwikkeling van de hersenen
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Single Cell Elektroporatie in vivo binnen de Intact ontwikkeling van de hersenen

Article DOI: 10.3791/705-v • 13:31 min • July 11th, 2008
July 11th, 2008 •
1,2, 1,2
1Brain Research Centre, University of British Columbia - UBC, 2Department of Cellular and Physiological Sciences, University of British Columbia - UBC

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Single-cell elektroporatie (SCE) is een gespecialiseerde techniek waardoor de levering van DNA of andere macromoleculen in individuele cellen in intact weefsel, ook in vivo preparaten. Hier hebben we detail de procedure voor SCE van een fluorescente kleurstof of plasmide DNA in neuronen in de intacte hersenen van de Xenopus laevis kikkervisje.

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Neurowetenschappen elektroporatie gen levering transfectie fluorescentie labeling neuronale beeldvorming micropipet
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