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Single Cell Elektroporation in vivo innerhalb der intakten sich entwickelnde Gehirn
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Single Cell Elektroporation in vivo innerhalb der intakten sich entwickelnde Gehirn

Article DOI: 10.3791/705-v • 13:31 min • July 11th, 2008
July 11th, 2008 •
1,2, 1,2
1Brain Research Centre, University of British Columbia - UBC, 2Department of Cellular and Physiological Sciences, University of British Columbia - UBC

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Einzel-Zell-Elektroporation (SCE) ist eine spezialisierte Technik, die Abgabe von DNA oder andere Makromoleküle in einzelne Zellen innerhalb intakten Gewebe, auch in vivo Präparate. Hier haben wir ausführlich das Verfahren für die SCE von einem fluoreszierenden Farbstoff-oder Plasmid-DNA in die Neuronen im intakten Gehirn des Xenopus laevis Kaulquappen.

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Neuroscience Ausgabe 17 die Elektroporation Gentransfer Transfektion Fluoreszenzmarkierung neuronale Bildgebung Mikropipette
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