The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
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Max Plank Institute for Molecular Cell Biology and Genetics, Dresden
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Bucholtz, F. Principles of Site-Specific Recombinase (SSR) Technology. J. Vis. Exp. (15), e718, doi:10.3791/718 (2008).
网站具体的重组（SSR）技术，使操纵基因的结构，探索基因功能和分子生物学已成为不可或缺的工具。网站，具体的重组酶的蛋白质结合，以不同的目标DNA序列。在1980年代在噬菌体的Cre / lox系统首次描述。 Cre重组是我型拓扑异构酶催化两个loxP位（X - OVER的P1位点）的网站站点特定的DNA重组。的Cre / lox系统不需要任何辅助因子。 LoxP位序列中包含不同的结合位点的Cre重组酶，环绕一个方向性的核心序列重组和重排的发生。当细胞中含有loxP位的网站，并表达Cre重组，重组事件发生。双链DNA Cre重组两个loxP位网站被切断，重新排列，并结扎（“剪刀和胶水”）。产品的重组事件的非对称序列的相对方向取决于。
SSR技术是经常使用的一种工具，以探讨基因的功能。这里感兴趣的基因表达Cre目标网站loxP位（“floxed”）两侧。动物，然后越过一个组织特异性启动子的控制下表达Cre重组酶的动物。组织中的表达Cre重组目标序列结合，并切除floxed基因。控制基因的缺失使基因在特定的组织和功能在不同的时间点的调查。采用SSR技术---有条件的突变的基因功能分析 - 已超过传统的敲奏通常是致命的基因缺失的显着优势。
Hi.
The presentation was good and substantial. It would much good if the delicate concepts of selection are also included.
Thank you.
Cheers,
Lopez Gilsinia Gilroy
1
ReplyPosted by: Lopez Gilsinia GilroyMay 31, 2008, 5:54 AM