Ricardo, R., Phelan, K. Counting and Determining the Viability of Cultured Cells. J. Vis. Exp. (16), e752, doi:10.3791/752 (2008).
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
The authors have nothing to disclose.