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단백질의 전기 영동 분리

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Proteomic Center, Keck Graduate Institute of Applied Life Sciences

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Cite this Article: 단백질의 전기 영동 분리

Chakavarti, B., Chakavarti, D. Electrophoretic Separation of Proteins. J. Vis. Exp. (16), e758, doi:10.3791/758 (2008).

Abstract: 단백질의 전기 영동 분리

전기는 단백질의 복잡한 혼합물 (예, 전지,​​ subcellular 분수, 열 분수, 또는 immunoprecipitates에서) 분리 subunit의 성분을 조사하고, 단백질 샘플의 균질성를 확인하는 데 사용됩니다. 그것은 또한 더 이상 응용 프로그램에서 사용하기 위해 단백질을 정화 제공할 수 있습니다. polyacrylamide 젤 전기 영동에서 단백질은 polyacrylamide 젤 매트릭스의 모공을 통해 전기 분야에 대한 응답으로 마이 그 레이션, 기공 크기는 증가 아크릴 아미드의 농도로 감소하게된다. 기공 크기와 단백질 요금, 크기 및 모양의 결합은 단백질의 마이 그 레이션 속도를 결정합니다. 이 단원에서는 표준 Laemmli 방법은 나트륨 dodecyl 황산 (SDS)의 면전에서, denaturing 조건 하에서 불연속 겔 전기 영동을위한 IE를 설명합니다.

Protocol: 단백질의 전기 영동 분리

이 실험 방법에 대한 전체 텍스트 프로토콜에서 사용할 수 있습니다 분자 생물학의 현재 프로토콜 .

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Disclosures: 단백질의 전기 영동 분리

Ask the Author: 단백질의 전기 영동 분리

5 Comments

Hi nice demo but I did not understand why the presenters kept on showing an agarose gel electrophoresed DNA gel in between as a representation instead of a protein gel.

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Posted by: swethaJune 15, 2008, 1:11 PM

SYPROorange and SYPROruby staining - two fluorescent-based protein detection methods - can appear like ethidium bromide stained DNA gels.  So those gels you are speaking of may actually be protein gels. 

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Posted by: Aaron K.June 16, 2008, 9:34 AM

Yeah possible,  but then why do we see only a single band.I guess SYPRO detection methods would detect the entire protein panel and not specific bands. unless otherwise they are detecting some specific enzyme activity using flourescent detection methods.

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Posted by: swethaJune 16, 2008, 9:52 AM

We loaded BSA.

Thanks.

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:12 AM

Please not that we showed polyacrylamide gel electrophoresis and not agarose gel electrophoresis.

Thanks.

Bulbul Chakravarti

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:08 AM

why cannot i see the video...? pls help me..

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Posted by: AnonymousOctober 10, 2008, 3:35 AM

i can see it now..thanks...

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Posted by: AnonymousOctober 10, 2008, 4:50 AM

my network is poor, I can not enjoy the video

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Posted by: Huang C.March 15, 2009, 6:30 AM

Hello,

Please shoot me an email at nikitab@jove.com and we'll figure something out for you.

Cheers,

Nikita

4.1

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Posted by: AnonymousMarch 22, 2009, 10:26 PM

Hi, thanks what can I download your video ? please help me

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Posted by: violet s.January 14, 2012, 10:35 AM

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