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电泳分离蛋白质

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Proteomic Center, Keck Graduate Institute of Applied Life Sciences

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Cite this Article: 电泳分离蛋白质

Chakavarti, B., Chakavarti, D. Electrophoretic Separation of Proteins. J. Vis. Exp. (16), e758, doi:10.3791/758 (2008).

Abstract: 电泳分离蛋白质

电泳用于分离复杂的蛋白质混合物(例如,从细胞,亚细胞组分,列分数,或免疫),调查亚基组成,并验证蛋白质样品的同质性。 ,它也可以用来净化在进一步的应用中使用的蛋白质。在聚丙烯酰胺凝胶电泳,蛋白质通过毛孔中的聚丙烯酰胺凝胶基质中的电场响应迁移;孔径丙烯酰胺浓度的增加而降低。孔径和蛋白质的电荷,大小和形状的组合决定了蛋白质的迁移率。在这个单元,标准的Laemmli方法描述不连续的凝胶电泳变性条件下,也就是说,在十二烷基硫酸钠(SDS),的存在。

Protocol: 电泳分离蛋白质

这个实验方法的完整的文本协议,可用在分子生物学中的电流协议。

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5 Comments

Hi nice demo but I did not understand why the presenters kept on showing an agarose gel electrophoresed DNA gel in between as a representation instead of a protein gel.

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Posted by: swethaJune 15, 2008, 1:11 PM

SYPROorange and SYPROruby staining - two fluorescent-based protein detection methods - can appear like ethidium bromide stained DNA gels.  So those gels you are speaking of may actually be protein gels. 

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Posted by: Aaron K.June 16, 2008, 9:34 AM

Yeah possible,  but then why do we see only a single band.I guess SYPRO detection methods would detect the entire protein panel and not specific bands. unless otherwise they are detecting some specific enzyme activity using flourescent detection methods.

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Posted by: swethaJune 16, 2008, 9:52 AM

We loaded BSA.

Thanks.

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:12 AM

Please not that we showed polyacrylamide gel electrophoresis and not agarose gel electrophoresis.

Thanks.

Bulbul Chakravarti

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:08 AM

why cannot i see the video...? pls help me..

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Posted by: AnonymousOctober 10, 2008, 3:35 AM

i can see it now..thanks...

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Posted by: AnonymousOctober 10, 2008, 4:50 AM

my network is poor, I can not enjoy the video

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Posted by: Huang C.March 15, 2009, 6:30 AM

Hello,

Please shoot me an email at nikitab@jove.com and we'll figure something out for you.

Cheers,

Nikita

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Posted by: AnonymousMarch 22, 2009, 10:26 PM

Hi, thanks what can I download your video ? please help me

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Posted by: violet s.January 14, 2012, 10:35 AM

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