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Proteinlerin Elektrogöçer Ayırma

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Proteomic Center, Keck Graduate Institute of Applied Life Sciences

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Cite this Article: Proteinlerin Elektrogöçer Ayırma

Chakavarti, B., Chakavarti, D. Electrophoretic Separation of Proteins. J. Vis. Exp. (16), e758, doi:10.3791/758 (2008).

Abstract: Proteinlerin Elektrogöçer Ayırma

Elektroforez, proteinlerin (örneğin, hücre, hücre içi kesirler, sütun kesirler, veya immunoprecipitates) kompleks karışımlar ayrı altbirim kompozisyonları araştırmak için, protein örneklerinin homojenliği ve doğrulamak için kullanılır. Ayrıca başka uygulamalar için protein arındırmak için hizmet edebilir. Poliakrilamid jel elektroforezi, proteinler poliakrilamid jel matris gözenekler yoluyla bir elektrik alanı yanıt göç; gözenek büyüklüğü artan akrilamid konsantrasyonu azalır. Gözenek boyutu ve protein yükü, boyut ve şekil kombinasyonu protein göç oranı belirler. Bu ünitede, standart Laemmli yöntemi, sodyum dodesil sülfat (SDS) varlığı, denatüre edici koşullar altında yani süreksiz jel elektroforezi için açıklanmıştır.

Protocol: Proteinlerin Elektrogöçer Ayırma

Bu deneysel bir yaklaşım için tam metin protokolü mevcuttur Moleküler Biyoloji Mevcut Protokoller .

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5 Comments

Hi nice demo but I did not understand why the presenters kept on showing an agarose gel electrophoresed DNA gel in between as a representation instead of a protein gel.

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Posted by: swethaJune 15, 2008, 1:11 PM

SYPROorange and SYPROruby staining - two fluorescent-based protein detection methods - can appear like ethidium bromide stained DNA gels.  So those gels you are speaking of may actually be protein gels. 

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Posted by: Aaron K.June 16, 2008, 9:34 AM

Yeah possible,  but then why do we see only a single band.I guess SYPRO detection methods would detect the entire protein panel and not specific bands. unless otherwise they are detecting some specific enzyme activity using flourescent detection methods.

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Posted by: swethaJune 16, 2008, 9:52 AM

We loaded BSA.

Thanks.

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:12 AM

Please not that we showed polyacrylamide gel electrophoresis and not agarose gel electrophoresis.

Thanks.

Bulbul Chakravarti

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Posted by: Bulbul ChakravartiJune 17, 2008, 2:08 AM

why cannot i see the video...? pls help me..

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Posted by: AnonymousOctober 10, 2008, 3:35 AM

i can see it now..thanks...

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Posted by: AnonymousOctober 10, 2008, 4:50 AM

my network is poor, I can not enjoy the video

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Posted by: Huang C.March 15, 2009, 6:30 AM

Hello,

Please shoot me an email at nikitab@jove.com and we'll figure something out for you.

Cheers,

Nikita

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Posted by: AnonymousMarch 22, 2009, 10:26 PM

Hi, thanks what can I download your video ? please help me

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Posted by: violet s.January 14, 2012, 10:35 AM

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