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Biology

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Molecular Evolution of the Tre Recombinase
 

Molecular Evolution of the Tre Recombinase

Article DOI: 10.3791/791-v 12:03 min May 29th, 2008
May 29th, 2008

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Summary

Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells. While still in its infancy, directed molecular evolution will allow the creation of custom enzymes that will serve as tools of molecular surgery and molecular medicine.

Tags

Molecular Evolution Tre Recombinase Directed Evolution HIV-1 Provirus Cre Recombinase LoxP Sequence LTR Sequences Genomic Sequences Recombination Activity Mutagenized Cre Libraries Directed Molecular Evolution Process Residual Activity Asymmetric LoxLTR Sequences Recombinase Libraries Reiterative Evolution Cycles Shuffled And Recombined Libraries Synergistic Mutations
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