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Van MEF tot Matrigel 3
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Van MEF tot Matrigel 3: passage uit hESC 's Matrigel op Matrigel

Article DOI: 10.3791/832-v • 03:51 min • June 10th, 2008
June 10th, 2008 •
1, 1, 1
1David Geffen School of Medicine, University of California, Los Angeles

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Deze video laat zien hoe het handhaven van de groei van menselijke embryonale stamcellen (hESC 's) in de feeder cel-vrije omstandigheden en hoe je continu passage hESC' s in de feeder cel-vrije omstandigheden. Bevestiging van hESC pluripotentie gegroeid in feeder celvrije voorwaarden door immunofluorescentie microscopie is ook aangetoond. Deel 3 van 3.

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Cellular Biology menselijke embryonale stamcellen (hESC) muis embryonale fibroblasten (MEF) Matrigel geconditioneerde-media feeder cel pluripotentie
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