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 JoVE Biology

Major Components of the Light Microscope

1

1Core Optical Imaging Facility, University of Texas Health Science Center at San Antonio (UTHSCSA)

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    Summary

    The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.

    Date Published: 7/30/2008, Issue 17; doi: 10.3791/843

    Cite this Article

    Centonze Frohlich, V. Major Components of the Light Microscope. J. Vis. Exp. (17), e843, doi:10.3791/843 (2008).

    Abstract

    The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.

    Protocol

    The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.

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    Disclosures

    The authors have nothing to disclose.

    Comments

    3 Comments

    do u llow downloads and if tes how do i do it.
    Reply

    Posted by: AnonymousSeptember 28, 2008, 8:10 AM

    Dear Sirs: We are Nikon distributors for Venezuela and we are watching some videos explicatives for microscopy that may help a lot to our users in order to learn better the theory of microscopy. We want to know if these videos can be downloadable or if we can set a link from our web site to yours in order to make our customers visit all these interesting visual material. Thanks for your comments, Claus PŒrner Commercial Director KO-BE Representaciones Científicas C.A. Caracas - Venezuela phone:58-²1²-²384²4²      
    Reply

    Posted by: AnonymousOctober 16, 2008, 9:52 AM

    I can hardley hear the dialogue.
    Reply

    Posted by: AnonymousDecember 18, 2008, 5:03 AM

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