Phase Contrast en differentieel interferentie contrast (DIC) Microscopie

I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?

I've seen that there we're colored images obtained using the DIC microscopy that looks like a 3-D image. Is there an approximation using DIC to measure the depth or height difference by just interpreting color differences?

In article abstract, the phase-contrast microscopy is claimed to have been invented in 1942. This is incorrect. The origins of phase-contrast date back to 1934 (not a microscopy yet) and 1935 (microscopy):

Zernike F. (1934)
Diffraction theory of the knife-edge test and its improved form, the phase-contrast method.
Monthly Notices of the Royal Astronomical Society 94 (5), 377-3­84 (originally published in Dutch).
(http://esoads.eso.org/journals_service.html)

Zernike F. (1935)
Phase-contrast method in microscopical observation (in German).
Physikalische Zeitschrift 36 (22/23): 848-851
Zeitschrift für Technische Physik 16 (11): 454-457
(two identical papers)

Furthermore, the following reports (published when Zernike was only 16 or 17 years old) utilize, in a way, the principle of phase-contrast microscopy, even though only for absorbing (i.e., not phase) objects:

Rheinberg J. (1904 and 1905)
On the influence on images of gratings of phase differences amongst their spectra.
J. Roy. Microsc. Soc.; volumes-not-numbered (Part 4), 388-390; and (Part 2), 152-155.
(www.biodiversitylibrary.org/bibliography/7413)

Conrady A. (1905)
An experimental proof of phase reversal in diffraction spectra.
J. Roy. Microsc. Soc.; volume-not-numbered (Part 2), 150-152.
(www.biodiversitylibrary.org/bibliography/7413)

Your presentation is excellent and locked in my neurons , the corresponding text in books do not give that effect

Nice video.
I may be mistaken, but one of the images (around 3:47) shown as DIC appears to be phase contrast.
Also, when mentioning setting up Koehler illumination during DIC (around 4:30), the video shows the alignment of phase rings. This is not needed (since you do not need to see the back focal plane of the microscope to establish Koehler) nor, in fact, what would be observed when setting up Koehler for DIC (there would be no phase annulus in the condenser if it is set up for DIC).

The video explained well what i was trying to learn in detail....
it is really nice
Thanks