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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

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Department of Entomology, Iowa State University

 

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Cite this Article: Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).

Abstract: Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

Protocol: Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.

Disclosures: Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

The authors have nothing to disclose.

Ask the Author: Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

1 Comment

I would like to know who sells the syringe-aplicator like you show in this video

Thank you

MV Miranda

1

Reply

Posted by: Maria V. MirandaDecember 19, 2008, 8:54 AM

The one used in these protocols was a Burkard Microprocessor Controller Pax-100.    I hope that will help you in your search.

 

W. O. Sparks

1.1

Reply

Posted by: Wendy SparksJanuary 29, 2009, 10:42 AM

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