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 JoVE General

Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

1, 1, 1

1Department of Entomology, Iowa State University

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    Summary

    In this video, we demonstrate two microapplicator techniques used to infect of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.

    Date Published: 8/23/2008, Issue 18; doi: 10.3791/889

    Cite this Article

    Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).

    Abstract

    Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

    Protocol

    Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.

    Disclosures

    The authors have nothing to disclose.

    Comments

    2 Comments

    I would like to know who sells the syringe-aplicator like you show in this video Thank you MV Miranda
    Reply

    Posted by: AnonymousDecember 19, 2008, 8:54 AM

    The one used in these protocols was a Burkard Microprocessor Controller Pax-100.    I hope that will help you in your search.   W. O. Sparks
    Reply

    Posted by: AnonymousJanuary 29, 2009, 10:42 AM

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