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 JoVE Biology

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

1, 1

1Department of Cell Biology, Emory University

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    Summary

    Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

    Date Published: 9/02/2008, Issue 19; doi: 10.3791/908

    Cite this Article

    Cross, M., Powers, M. In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts. J. Vis. Exp. (19), e908, doi:10.3791/908 (2008).

    Abstract

    Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

    Protocol

    The complete text protocol for this experimental approach is available in Current Protocols in Cellular Biology.

    Disclosures

    The authors have nothing to disclose.

    Comments

    2 Comments

    is good congratulations for you job, i am mexico
    Reply

    Posted by: AnonymousSeptember 3, 2008, 12:26 AM

    How to see references???
    Reply

    Posted by: AnonymousSeptember 7, 2008, 10:08 PM

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