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The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.
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Department of Neurology and Neurological Sciences, Stanford University School of Medicine
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Taniguchi, H., Andreasson, K. The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia. J. Vis. Exp. (21), e955, doi:10.3791/955 (2008).
缺氧缺血性脑病（HIE）是全身出生时发生窒息的后果。二十五％的新生儿缺氧缺血性脑病的发展严重和永久性的神经心理的后遗症，包括精神发育迟滞，脑瘫，癫痫。缺氧缺血性脑病的结果是灾难性的，永久的，使得它的关键识别和制定治疗策略，以减少新生儿缺氧缺血性脑病的脑损伤。为此，新生儿缺氧缺血性脑损伤大鼠模型已开发模型这个人的条件。 Vannucci等1缺氧缺血性脑病模型首次验证，并一直广泛用于识别从围产期缺氧缺血导致的脑损伤的机制，来测试潜在的治疗干预3,4。缺氧缺血性脑病模型是一个两步的过程，涉及暴露在缺氧的环境，左颈总动脉结扎。半球同侧颈动脉结扎脑血流（CBF）的不减少，因为通过Willis环侧支血流;然而，随着氧张力降低，CBF在同侧半球显着降低和单方面缺血性损伤的结果。使用2,3,5 -三苯基氯化四氮唑（TTC）染色，并确定脑组织缺血，最初是成年啮齿动物脑缺血5模型，用于评估在早期的时间点脑infarctin的程度高达72小时后缺血事件6。在这段视频中，我们展示了在产后大鼠脑缺氧缺血损伤模型和使用TTC染色梗死大小的评价。
批准该协议是在斯坦福大学和遵守机构的动物护理和使用委员会由国家机构使用实验动物的健康指引。
新生大鼠缺氧缺血性脑病模型
代表性的成果
图1。代表缺氧缺血性脑病冠状水平1-4与TTC染色。
TTC染色冠状切片显示梗死区（白）。
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产后缺氧缺血性脑病的啮齿类动物模型建立的模型，概括脑缺氧，在新生儿围产后期间发生。广泛的组织学和免疫特性的研究表明，7天的老灭鼠有类似的大脑成熟模型孕晚期胎儿7。鼠缺氧缺血性脑病模型已经非常丰富，了解脑损伤的围产后缺氧，低温干预措施，如已证实 3机制。这个模型最初是完善在大鼠和小鼠8最近改编。
值得一提的有具体的技术细节：
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由美国心脏协会和March of Dimes的。
| Name | Type | Company | Catalog Number | Comments |
| Sprague-Dawley Rat Pups | Animal | Charles River Laboratories | ||
| Isoflurane | Surgery | Baxter Internationl Inc. | ||
| 8% Oxygen/ 92% Nitrogen Gas | Surgery | Airgas | ||
| 2,3,5-triphenyl tetrazolium chloride | Reagent | Sigma-Aldrich | T8877 | |
| Phosphate buffered saline (PBS) pH 7.4 | Reagent | GIBCO, by Life Technologies | ||
| Paraformaldehyde | Reagent | Sigma-Aldrich | P6148 |
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Thanks a lot! Different lab do HIE model have many difference.We put pups in container and container was put in baby incubator to keep the temperature inside the container with 37 degree.
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ReplyPosted by: Zhiheng HuangDecember 14, 2008, 2:18 AM