Sydney Medical School 1 article published in JoVE Biochemistry An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants Hannes Steinkellner1, Alexander V. Beribisky1, Philip Mausberg1, John Christodoulou2, Barbara Scheiber-Mojdehkar3, Anna Huber1, Victoria Sarne1, Franco Laccone1 1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), 2Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, 3Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.