The Affiliated Hospital of Hangzhou Normal University 1 article published in JoVE Genetics In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions Lina Yu*1, Wenxiu He*1, Jie Xie*1, Rui Guo*1, Juan Ni2, Xia Zhang3, Quishi Xu1, Caifeng Wang1, Qiuling Yue1, Fangfang Li1, Mengcheng Luo4, Bo Sun3, Lan Ye1, Ke Zheng1 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions.