Guangzhou University of Chinese Medicine 7 articles published in JoVE Medicine Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model Po-En Chiu*1,2, Zhonghua Fu*3,4, De-Wei Lai5,6, Li-Wei Chou7,8,9 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang Bing Show Chwan Memorial Hospital, 6Department of Pharmacy and Master Program, Tajen University, 7Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 8Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 9Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats. Medicine Management of the Uncinate Process in No-Touch Laparoscopic Pancreaticoduodenectomy Zhantao Shen1,2, Xiang Wu1,2, Fanxing Huang2, Guihao Chen1,2, Yifeng Liu1,2, Zhimin Yu1,2, Chunbao Zhu1,2, Zhijian Tan1,2, Xiaosheng Zhong1,2 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine Complete resection of the uncinate process and mesopancreas is one of the most important and difficult processes in laparoscopic pancreatoduodenectomy (LPD). This article presents a method for managing the uncinate process in no-touch LPD using the median-anterior and left-posterior approaches to the superior mesenteric artery (SMA). Medicine Fu's Subcutaneous Needling for Knee Osteoarthritis Pain Po-En Chiu*1,2, Zhonghua Fu*3,4, Jian Sun4,5,6, Guan-Wei Jian7,8, Te-Mao Li9, Li-Wei Chou10,11,12 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills. Neuroscience Dual-Task Stroop Paradigm for Detecting Cognitive Deficits in High-Functioning Stroke Patients Songjun Lin1,2, Qiang Lin3,4,5, Biyi Zhao3,4,6, Yongchun Jiang3,4, Wanqi Zhuang3,4, Delong Chen3,4, Yajie Zhang3,4, Aijia Chen3,4, Qianrong Zhang7, Yuxin Zheng3,4, Jianjun Wang1, Fangqiu Xu8, Xi Qin3,4,9, Yefeng Cai10 1Department of Neurology and Psychology, the Fourth Clinical Medical College, Guangzhou University of Chinese Medicine, Shenzhen Traditional Chinese Medicine Hospital, 2Guangzhou University of Chinese Medicine, 3Department of Rehabilitation Medicine, The Fifth Affiliated Hospital of Guangzhou Medical University, 4Department of Rehabilitation Medicine, Guangzhou Medical University, 5Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, 6School of Traditional Chinese Medicine, Jinan University, 7Health College of Guangdong Pharmaceutical University, 8Department of Clinical Medicine, Guangzhou Medical University, 9Department of Rehabilitation Medicine, Panzhihua Central Hospital, 10Department of Neurology and Psychology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine Clinical assessment scales are notsensitive enough to cognitive dysfunction in high-functioning stroke patients. The dual-task paradigm presents advantages and potential in the assessment and cognitive training of cognitive dysfunction. The study here proposes a dual-task Stroop paradigm to identify cognitive dysfunction in high-functioning stroke patients. Medicine Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique Zhantao Shen1,2, Xiang Wu1,2, Guihao Chen1,2, Yifeng Liu1,2, Chunbao Zhu2, Fanxing Huang2, Zhijian Tan1,2, Xiaosheng Zhong1,2 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 2The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine No-Touch isolation procedures might prevent the dissemination of cancer cells from the primary tumor. However, these techniques are not widely accepted in laparoscopic pancreatoduodenectomy (LPD) by now. We herein present in-situ No-Touch isolation LPD with partial resection and reconstruction of the superior mesenteric vein (SMV) for pancreatic cancer after neoadjuvant therapy. Neuroscience Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder Rui Wang1, Zi-tong Huang3, Wen-kang Ren1, Jiao Zhang1, Yao Zhang1, Bo Tan3, Ping Huang1,2, Hong-ying Cao1,2 1School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, 2Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine, Guangzhou University of Chinese Medicine, 3School of Basic Medical Sciences, Guangzhou University of Chinese Medicine This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments. Medicine Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine Yalan Wu*1, Shengjun Wu*1, Xin-an Huang1, Qingping Zeng1, Tao Deng1, Fang Liu1 1Institute of Tropical Medicine, Guangzhou University of Chinese Medicine This protocol describes a novel colorimetric method for antimalarial primaquine (PMQ) detection in synthetic urines and human serums.
