Key Laboratory of Gastrointestinal Cancer (Fujian Medical University) 1 article published in JoVE Cancer Research Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy Hansjörg Habisch*1, Fangrong Zhang*1,2, Qishun Zhou1, Tobias Madl1 1Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Molecular Biology and Biochemistry, Medical University of Graz, 2Ministry of Education, School of Basic Medical Sciences, Key Laboratory of Gastrointestinal Cancer (Fujian Medical University) The present protocol describes the preparation and quantitative measurement of free and protein-bound arginine and methyl-arginines by 1H-NMR spectroscopy.