National Centre for Biological Sciences View Institution's Website 2 articles published in JoVE Behavior Tracking Sugar-Elicited Local Searching Behavior in Drosophila Manal Shakeel1,2, Shivam Kaushik3, Teiichi Tanimura4, Axel Brockmann1, Pinky Kain3,5 1National Centre for Biological Sciences, 2The University of Trans-Disciplinary Health Sciences and Technology, 3Regional Centre for Biotechnology, 4Neural Circuit Group, Division of Biological Science, Graduate School of Science, Nagoya University, 5Perelman School of Medicine, University of Pennsylvania This protocol describes a behavioral assay for recording sugar-elicited search behavior using Drosophila melanogaster. The assay can be utilized to study feeding and foraging-related behaviors, as well as the underlying neuronal mechanisms. Bioengineering Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish Sudip Mondal1, Shikha Ahlawat1, Sandhya P. Koushika1,2 1Neurobiology, NCBS-TIFR, 2Department of Biological Sciences, TIFR A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.
