Indian Oil Corporation, Research and Development 1 article published in JoVE Environment Enhanced Oil Recovery using a Combination of Biosurfactants Arif Nissar Zargar1, Nidhi Patil1, Manoj Kumar2, Preeti Srivastava1 1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, 2Indian Oil Corporation, Research and Development Faridabad We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.