The Islamic University 1 article published in JoVE Engineering Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis Ateeq ur Rehman1, Rahman S. Zabibah2, Samira Kharratian3, Adil Mustafa4,5 1Department of Biomedical Engineering, Foundation University Islamabad, 2College of Medical Technology, The Islamic University Najaf, 3Faculty of Mechanical Engineering, University of Tabriz, 4Department of Engineering Mathematics, University of Bristol, 5School of Life Sciences, University of Warwick Breast cancer cells exhibit different dielectric properties compared to non-tumor breast epithelial cells. It has been hypothesized that, based on this difference in dielectric properties, the two populations can be separated for immunotherapy purposes. To support this, we model a microfluidic device to sort MCF-7 and MCF-10A cells.