Inha University 3 articles published in JoVE Engineering Development of a Nose-only Inhalation Toxicity Test Chamber That Provides Four Exposure Concentrations of Nano-sized Particles Jae-Seong Yi1,2, Ki-Soo Jeon2, Hee-Jung Kim2, Ki-Joon Jeon1, Il-Je Yu2 1Department of Environmental Engineering, Inha University, 2HCTM Co. Ltd A nose-only inhalation toxicity chamber capable of testing inhalation toxicity at four different exposure concentrations was designed and validated for flow field uniformity and cross-contamination between the exposure ports for each concentration. Here, we present a protocol to confirm that the designed chamber is effective for inhalation toxicity testing. Engineering Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte Healin Im*1, Seok Hwan Jeong*1, Dong Hyuk Park2, Sunkook Kim1, Young Ki Hong1 1School of Advanced Materials Science and Engineering, Sungkyunkwan University, 2Department of Applied Organic Materials Engineering, Inha University A protocol for fabricating nanoporous anodic aluminum oxides via simultaneous multi-surfaces anodization followed by stair-like reverse biases detachments is presented. It can be applied repeatedly to the same aluminum substrate, exhibiting a facile, high-yield, and environmentally clean strategy. Bioengineering Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film Sangbaek Choi1,3, Sunhee Yoon1,3, Hyunil Ryu1,3, Sun Min Kim2,3,4, Tae-Joon Jeon1,3,4 1Department of Biological Engineering, Inha University, 2Department of Mechanical Engineering, Inha University, 3Biohybrid Systems Research Center (BSRC), Inha University, 4Convergent Research Center for Metabolism and Immunoregulation (CRCMI), Inha University We demonstrate a storable, transportable lipid bilayer formation system. A lipid bilayer membrane can be formed within 1 hr with over 80% success rate when a frozen membrane precursor is brought to ambient temperature. This system will reduce laborious processes and expertise associated with ion channels.