The Heart Research Institute View Institution's Website 4 articles published in JoVE Biology Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing Jasmine Jin1, Haoqing Jerry Wang1,2,3, Yiyao Catherine Chen1, Blake Russell1, Allan Sun1,2,3,4, Yao Wang1, Lining Arnold Ju1,2,3,4 1School of Biomedical Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4The University of Sydney Nano Institute (Sydney Nano), The University of Sydney The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology. Bioengineering Molecular Spring Constant Analysis by Biomembrane Force Probe Spectroscopy Peyman Obeidy1, Haoqing Wang1,2,3, Mingqin Du1, Huiqian Hu1,4, Fang Zhou1, Haoruo Zhou5, Hao Huang5, Yunduo Charles Zhao1,2, Lining Arnold Ju1,2,3 1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney A biomembrane force probe (BFP) is an in situ dynamic force spectroscopy (DFS) technique. BFP can be used to measure the spring constant of molecular interactions on living cells. This protocol presents spring constant analysis for molecular bonds detected by BFP. Immunology and Infection In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages Yunjia Zhang1,2, Benjamin S. Rayner1,2, Mathias Jensen3, Clare L. Hawkins1,2,3 1Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Department of Biomedical Sciences, University of Copenhagen Presented here is a protocol to detect macrophage extracellular trap (MET) production in live cell culture using microscopy and fluorescence staining. This protocol can be further extended to examine specific MET protein markers by immunofluorescence staining. Medicine Murine Model of Wound Healing Louise Dunn1,2, Hamish C. G Prosser1,2, Joanne T. M. Tan1,2, Laura Z. Vanags1,2, Martin K. C. Ng1,2,3, Christina A. Bursill1,2 1The Heart Research Institute, 2Sydney Medical School, University of Sydney, 3Cardiology Department, Royal Prince Alfred Hospital A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.