Ingham Institute 2 articles published in JoVE Immunology and Infection Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection Thomas Tu1,2, Stephan Urban1,3 1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory, Ingham Institute, 3German Center for Infection Research (DZIF) We describe here the in vitro generation of HBV DNA via a Hepatitis B virus infection system and the highly sensitive detection of its (1–2 copies) integration using inverse nested PCR. Bioengineering Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles Murray C. Killingsworth1,2,3,4, Yuri V. Bobryshev3,4,5 1South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, 2School of Medicine, Western Sydney University, 3Correlative Microscopy Group, Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.