Izon Science 1 article published in JoVE Bioengineering Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example Emma L. C. J. Blundell1, Robert Vogel2,3, Mark Platt1 1Department of Chemistry, School of Science, Loughborough University, 2Izon Science Limited, 3School of Mathematics and Physics, The University of Queensland Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles.